Uniprot-lamin-a, lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. lamin a and c are present in equal amounts in the lamina of mammals. plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics.. « hide 10 20 30 40 50 metpgqkrat rsthtplspt ritrlqeked lqglndrlav yidkvrslel 60 70 80 90 100 enarlrlrit esedvisrev tgiksayete ladarktlds vakerarlql 110 120 130 140 150 elskireehk elkarnakke sdlltaqarl kdleallnsk daalttalge 160 170 180 190 200 krnleneire lkahiaklea sladtkkqlq demlrrvdte nrnqtlkeel 210 220 230 240 250 efqksiynee mretkrrhet rlvevdngrq refesklada lhelraqheg 260 270 280 290 300 ..., western blot analysis of extracts from control hela cells (lane 1) or hela cells with an apparent in-frame truncation mutation in the gene encoding lmna (lane 2) using lamin a/c antibody #2032 (upper) or β-actin (d6a8) rabbit mab #8457 (lower)..
A specific mutation in the lmna gene has been found in most patients with hutchinson-gilford progeria syndrome, which is a condition that causes the dramatic, rapid appearance of aging beginning in childhood. this mutation changes a single dna building block (nucleotide) in the gene. specifically, the mutation replaces the nucleotide cytosine with the nucleotide thymine at position 1824 ..., dam id n6-methyladenine (m6a) is the product of the addition of a methyl group (ch 3) at position 6 of the adenine. this modified nucleotide is absent.
Cell culture. dld-1 colorectal adenocarcinoma cells were obtained from the laboratory of thomas ried (nci, nih, bethesda, usa). these cells were maintained in rpmi media (invitrogen, rpmi 1640, 11875-093) supplemented with 10% fetal bovine serum (fbs, invitrogen, 6140-079 carlsbad, usa) and antibiotics—penicillin (100 u/ml) and streptomycin (100 μg/ml, invitrogen, 15070-063) at 37°c with 5 ..., introduction. the cytoskeleton perceives and relays altered extracellular forces into the nucleus in order to regulate growth, development and differentiation ().the linc (linker of nucleoskeleton and cytoskeleton) complex communicates extracellular forces into the nucleus via cytoskeletal proteins on the cytoplasmic side and lamins at the inner nuclear membrane..
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